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https://en.wikipedia.org — Eighteen Soon after three washes inside PBST, the actual coverslips had been incubated using fluorescein isothiocyanate�Cconjugated anti-rabbit IgG (AffiniPure, One:Two hundred; Knutson ImmunoResearch, Gulf Grove, Pennsylvania) regarding One hour at 70 degrees and counterstained using 2.5 ��g/mL bisbenzimide (Hoechst color 33258; Sigma-Aldrich, St. Louis, MO; Osaka, Okazaki, japan). In ord
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